TIMTEC-BB SBB001081
Procaine hydrochloride
CAS: 51-05-8
Molecular Formula: C13H21ClN2O2
TIMTEC-BB SBB001081 - Names and Identifiers
| Name | Procaine hydrochloride |
| Synonyms | cetain irocaine eugerase ethocaine bernocaine ATOXICAINE chlorocaine Procaine Hcl TIMTEC-BB SBB001081 Procaine hydrochloride 2-(Diethylamino)ethyl 4-aminobenzoate 2-methylpropyl 4-aminobenzoate hydrochloride diethylaminoethanol4-aminobenzoatehydrochloride |
| CAS | 51-05-8 |
| EINECS | 200-077-2 |
| InChI | InChI=1/C13H20N2O2.ClH/c1-4-15(5-2)13(3,17)12(16)10-6-8-11(14)9-7-10;/h6-9,17H,4-5,14H2,1-3H3;1H |
| InChIKey | HCBIBCJNVBAKAB-UHFFFAOYSA-N |
TIMTEC-BB SBB001081 - Physico-chemical Properties
| Molecular Formula | C13H21ClN2O2 |
| Molar Mass | 272.77 |
| Density | 1.1761 (rough estimate) |
| Melting Point | 155-156°C(lit.) |
| Boling Point | 195-196°C 17mm |
| Flash Point | 195-196°C/17mm |
| Water Solubility | soluble |
| Solubility | Solution S is clear (2.2.1) and colourless (2.2.2, Method II). |
| Appearance | Crystals or Crystalline Powder |
| Color | Colorless to white |
| Merck | 14,7757 |
| BRN | 3917802 |
| Storage Condition | 2-8°C |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Sensitive | Air Sensitive |
| Refractive Index | 1.5270 (estimate) |
| Use | Local anesthetics for infiltration of local anesthesia, nerve block, etc |
| In vitro study | Procaine acts primarily by inhibiting the influx of sodium ions through voltage-gated sodium channels on the cell membrane of neurons in peripheral nerves. When the internal flow of sodium ions is interrupted, the action potential cannot occur and the signal transmission is therefore inhibited. The receptor site is considered to be located in the cytoplasmic (internal) portion of the sodium channel. Procaine is also capable of binding to or antagonizing the function of the N-methyl-D-aspartate (NMDA) receptor and the nicotinic acetylcholine receptor and serotonin receptor-ion channel complex. Procaine is an inhibitor of calcium and caffeine-induced calcium release in various types of muscle preparations. 0.5 mM Procaine blockade of sarcoplasmic reticulum in individual lipid bilayers CaProcaine can be used as a DNA demethylating agent with inhibitory effect on the growth of human cancer cells. 0.5 mM Procaine was able to reduce 5-methylcytosine DNA by 40% in MCF-7 breast cancer cell lines. Procaine is also able to bind to CpG-enriched DNA, demethylating densely hypermethylated CpG sequences, resulting in epigenetically silenced genes restoring gene expression. Procaine treatment (0.5 mM) increased the mitotic index of cells in M-phase. Procaine treatment (1 mM) reduced cell proliferation by ~ 40%. Procaine affects the shape and deformability of red blood cells. 45 mM Procaine almost completely prevented the discoid cell-Acanthocyte conversion associated with ATP depletion. Similar concentrations of Procaine normalized ATP-depleted cell stickiness and filterability, but had no effect on cell volume, osmotic fragility, or monovalent cation composition. |
| In vivo study | Procaine is a stimulant of the cells of the limbic system. In awake cats, 15 mg/kg Procaine increased cellular activity in the hippocampus ventral amygdala, nucleus accumbens, temporal neocortex, and ventromedial hypothalamus. Procaine promotes the conduction of evoked stimulatory activity from the amygdala to the ventromedial hypothalamus. sup> [8]Procaine affects the frequency and amplitude of rhythmic slowing activity in the hippocampus elicited as a network. Procaine (0.5 microliters, 20% weight/volume) is injected in the ascending system before the suprapillary nucleus, near the medial forebrain bundle or in the medial barrier area, it reduces the amplitude of hippocampal rhythmic slowing activity (RSA) elicited in the form of a reticular, with no effect on frequency. The injection of Procaine in the ascending system preceding the site of reticular stimulation, reaching, and including the suprapapillary nucleus, simultaneously reduced the frequency and amplitude of RSA elicited in the reticular form. In rats, Procaine (80 mg/kg) increased the duration and conduction range of post-epileptiform discharges (ADs) produced by electrical stimulation of the amygdala. Porcaine also increased seizure development (kindling) in rats produced by repeated amygdala stimulation. In adequately stimulated subjects, Procaine may also act as a convulsant. Procaine produced a weak but significant increase in the amplitude of the transcallosal evoked potentials. Procaine affects the production of auditory brainstem responses (ABRs). Procaine (30 microliters of 1% solution) was injected into the oblique body of guinea pigs, affecting many scalp-derived ABR components: N2 was delayed, the duration of P2 was longer, P3 and N3 disappeared, the latency of P4 is shortened, the duration becomes longer, the amplitude is not affected, and N4 is greatly attenuated. Only P1 and N1 were not affected by procaine injection. Procaine increases the therapeutic index of cisplatin by improving the antitumor activity of cisplatin and reduces its nephrotoxicity. In BDF1 mice, the combined administration of cisplatin and Procaine (40 mg/kg) produced 50% of the lethal dose (LD50) and 90% of the lethal dose (LD90) the value is almost 2-fold higher than that of cisplatin alone. The combined administration produced a higher cure rate than cisplatin alone (50% vs. 9%). After 4-7 days of cisplatin alone, increased blood urea nitrogen (BUN) levels were observed, as well as tubular degeneration detected by light microscopy, these phenomena were not observed when the same doses of cisplatin and Procaine were administered in combination. |
TIMTEC-BB SBB001081 - Risk and Safety
| Hazard Symbols | T - Toxic |
| Risk Codes | R25 - Toxic if swallowed R43 - May cause sensitization by skin contact R36/37/38 - Irritating to eyes, respiratory system and skin. R23/24/25 - Toxic by inhalation, in contact with skin and if swallowed. |
| Safety Description | S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S37/39 - Wear suitable gloves and eye/face protection S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| UN IDs | UN 2811 6.1/PG 3 |
| WGK Germany | 3 |
| RTECS | DG2275000 |
| TSCA | Yes |
| HS Code | 29242990 |
| Hazard Class | 6.1 |
| Packing Group | III |
| Toxicity | LD50 in mice (mg/kg): 660 ± 60 s.c. (Goldenthal) |
TIMTEC-BB SBB001081 - Standard
Authoritative Data Verified Data
This product is 4-aminobenzoic acid -2-(diethylamino) ethyl ester hydrochloride. The content of C13H20N202 • HC1 shall not be less than 99.0% calculated as dry product.
Last Update：2024-01-02 23:10:35
TIMTEC-BB SBB001081 - Trait
Authoritative Data Verified Data
- This product is white crystal or crystalline powder; Odorless.
- This product is soluble in water, slightly soluble in ethanol, slightly soluble in chloroform, almost insoluble in ether.
melting point
The melting point of this product (General rule 0612 first method) is 154~157°C.
Last Update：2022-01-01 15:35:08
TIMTEC-BB SBB001081 - Differential diagnosis
Authoritative Data Verified Data
- take about 0.lg of this product, add 2ml of water to dissolve, add 1ml of 10% sodium hydroxide solution to generate white precipitate; Heat, turn into oily matter; Continue heating, the generated vapor can make the wet red litmus test paper turn blue; Heat to the oil disappeared after cooling, plus hydrochloric acid acidification, that is, the precipitation of white precipitate.
- The infrared absorption spectrum of this product should be consistent with that of the control (Spectrum set 397).
- the aqueous solution of this product was chloride identification (1) of the reaction (General 0301).
- This product shows the identification reaction of aromatic first amines (General rule 0301).
Last Update：2022-01-01 15:35:08
TIMTEC-BB SBB001081 - Exam
Authoritative Data Verified Data
acidity
take 0.40g of this product, Add 10ml of water to dissolve, add 1 drop of Methyl red indicator solution, if it is red, add 0.02 ml of sodium hydroxide titration solution (0.20 mol/L), and the strain is orange.
clarity of the solution
take 2.0g of this product and add 10ml of water to dissolve. The solution should be clarified.
p-aminobenzoic acid
take this product, precision weighing, adding water to dissolve and quantitatively dilute to make a solution containing 0.2mg per lml, as a test solution; Take the other amino benzoic acid reference substance, precision weighing, water is added to dissolve and quantitatively dilute to prepare a solution containing 1ug per 1 ml, which is used as a reference solution; 1 ml of the test solution and 9ml of the reference solution are mixed uniformly as a system applicable solution. High performance liquid chromatography (General 0512) test, using eighteen alkyl silane bonded silica gel as filler; 0.1% mol / L potassium dihydrogen phosphate solution containing 0.05 sodium heptane sulfonate (pH adjusted to 3.0 with phosphoric acid)-Methanol (68:32) as mobile phase; Detection wavelength of 279nm. Take 10ul of the applicable solution of the system and inject it into human liquid chromatograph. The number of theoretical plates shall not be less than 2000 according to the peak of p-aminobenzoic acid. The separation degree of procaine peak and p-aminobenzoic acid peak shall be greater than 2.0. 10u1 of the reference solution and the test solution were respectively injected into the liquid chromatograph, and the chromatograms were recorded. If there is a chromatographic peak in the chromatogram of the test solution that is consistent with the retention time of the p-aminobenzoic acid peak, the peak area shall be calculated according to the external standard method, and shall not exceed 0.5%.
loss on drying
take this product, dry to constant weight at 105°C, weight loss shall not exceed 0.5% (General rule 0831).
ignition residue
take l.Og of this product and check it according to law (General rule 0841). The residue left shall not exceed 0.1%.
Iron Salt
take the residue left under the item of ignition residue, add 2ml of hydrochloric acid, steam dry on a water bath, add 4ml of dilute hydrochloric acid, dissolve at a slight temperature, add 30ml of water and 50mg of ammonium persulfate, inspection according to law (General rule 0807), compared with the standard iron solution made of 0.001% of the control solution, not deeper ().
Heavy metals
take 2.0g of this product, add 15ml of water to dissolve, add 2ml of acetate buffer (pH 3.5) and an appropriate amount of water to make 25ml, and check according to law (General Principles 0821 first law), heavy metals should not be more than 10 parts per million.
Last Update：2022-01-01 15:35:09
TIMTEC-BB SBB001081 - Content determination
Authoritative Data Verified Data
take this product about 0.6g, precision weighing, according to the permanent stop titration method (General 0701), at 15~25°C, with sodium nitrite titration solution (0.lmol/L) titration. Each 1 ml of sodium nitrite titration solution (0.1 mol/L) corresponds to 27.28mg of C13H20N2O2 · hc1.
Last Update：2022-01-01 15:35:09
TIMTEC-BB SBB001081 - Category
Authoritative Data Verified Data
local anesthetic.
Last Update：2022-01-01 15:35:10
TIMTEC-BB SBB001081 - Storage
Authoritative Data Verified Data
light shielding, sealed storage.
Last Update：2022-01-01 15:35:10
TIMTEC-BB SBB001081 - Procaine Hydrochloride Injection
Authoritative Data Verified Data
This product is an isotonic sterile aqueous solution containing an appropriate amount of sodium aerated procaine hydrochloride. Procaine hydrochloride (C13H20N202 • HC1) shall be included at 95.0% to 105.0% of the label load.
trait
This product is a clear colorless liquid.
identification
- take this product, according to the item of procaine hydrochloride identification (3), (4) test, showed the same reaction.
- in the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the reference solution.
- take this product (about 80mg equivalent to procaine hydrochloride), water bath evaporation, residual submergence and vacuum drying, determination according to law. The infrared absorption spectrum of this product should be consistent with the spectrum of the control (Spectrum set 397 figure).
examination
- the pH value should be 3.5 to 5.0 (General 0631).
- precise measurement of related substances: take an appropriate amount of this product and quantitatively dilute it with water to make a solution containing about 0.2mg of procaine hydrochloride per lml as a test solution; Take lml for precise measurement, put it in a 100ml measuring flask, dilute it with water to the scale, shake well, and use it as a control solution; Take an appropriate amount of P-aminobenzoic acid reference substance, weigh it precisely, add water to dissolve and quantitatively dilute it to make a solution containing about 2.4ug per 1 ml, as a reference solution; Take the test solution 1 ml and the control solution 9ml mixed evenly, as the system applicable solution. According to the method under the item of procaine hydrochloride and p-aminobenzoic acid, take the reference solution, the control solution and the test solution with 10 u1 respectively, and inject the human liquid chromatograph respectively, the chromatogram was recorded to 4 times the retention time of the main component peak. If there is a chromatographic peak in the chromatogram of the test solution that is consistent with the retention time of the peak of p-aminobenzoic acid, the peak area shall be calculated according to the external standard method, and 1.2% of the labeled amount of procaine hydrochloride shall not be passed, the sum of the peak areas of other impurities shall not be greater than the main peak area of the control solution (1.0%).
- osmolality this product shall be taken and checked according to law (General rule 0632). The osmolality ratio shall be 0.9-1.1.
- bacterial endotoxin take this product, available 0.06EU / ml above the high sensitivity of the Limulus reagent, according to the law (General 1143), per 1 mg of procaine hydrochloride containing endotoxin should be less than 0.20EU.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
- measured by high performance liquid chromatography (General 0512).
- chromatographic conditions and system suitability test using eighteen alkyl silane bonded silica gel as filler; 0.1% mol / L potassium dihydrogen phosphate solution containing 0.05 sodium heptane sulfonate (adjusted to pH 3.0 with phosphoric acid)-Methanol (68:32) as mobile phase; The detection wavelength was 290mn, and the number of theoretical plates was not less than 2000 based on the procaine peak. The degree of separation of procaine peaks from adjacent impurity peaks shall meet the requirements.
- determination precision: take an appropriate amount of this product and quantitatively dilute it with water to make a solution containing 0.02mg of procaine hydrochloride per 1 ml as a test solution, and inject 10ul into the liquid chromatograph for precision measurement, record the chromatogram; Take the procaine hydrochloride reference, precision weigh, add water to dissolve and quantitatively dilute to make a solution containing 0.02mg procaine hydrochloride in 1 ml, and determine with the same method. According to the external standard method to calculate the peak area, that is.
category
with procaine hydrochloride.
specification
(l)2ml:40mg (2) 10ml:lOOmg (3)20ml:50mg (4)20ml:lOOmg
storage
light shielding, closed storage.
Last Update：2022-01-01 15:35:11
TIMTEC-BB SBB001081 - Procaine hydrochloride for injection
Authoritative Data Verified Data
This product is a sterile powder of procaine hydrochloride. The content of procaine hydrochloride (C13H20N202 • HCl) shall be between 95.0% and 105.0% of the labeled amount calculated as the average loading.
trait
This product is white crystal or crystalline powder; Odorless.
identification
take this product, according to the item of procaine hydrochloride identification (1), (3), (4) test, show the same reaction.
examination
- acidity: take 0.40g of this product, Add 10ml of water to dissolve, add 1 drop of Methyl red indicator solution, if it is red, add 0.02 ml of sodium hydroxide titration solution (0.20 mol/L), the strain was orange.
- the clarity of the solution take this product 2.0g, add water 10ml dissolved, the solution should be clear.
- the content of p-aminobenzoic acid under the item of difference in loading amount is appropriately weighed, accurately weighed, dissolved with water and quantitatively diluted to prepare a solution containing 0.2mg of procaine hydrochloride per 1 ml as a test solution; the p-aminobenzoic acid reference substance was accurately weighed, dissolved by adding water and quantitatively diluted to prepare a solution containing lug of p-aminobenzoic acid per 1 ml as a reference solution. According to the determination method under the item of p-aminobenzoic acid in procaine hydrochloride, if there is a chromatographic peak in the chromatogram of the test solution that is consistent with the retention time of p-aminobenzoic acid peak, the peak area shall be calculated according to the external standard method, not 0.5% of the labeled amount of procaine hydrochloride.
- weight loss on drying this product shall be dried at 105°C to constant weight, and the weight loss shall not exceed 1.0% (General rule 0831).
- bacterial endotoxin this product, according to the method of procaine hydrochloride injection under the inspection, should comply with the provisions.
- sterile take this product, respectively, plus sterile water made in each lml containing 30mg of the solution, according to the law (General Principles 1101), should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
Content determination
take the contents under the difference of loading amount, mix evenly, weigh an appropriate amount (equivalent to 0.6g of procaine hydrochloride) accurately, and follow the permanent stop titration method (General rule 0701), at 15-25 ° C, with sodium nitrite titration solution (0.lmol/L) titration. Each 1 ml of sodium nitrite titration solution (0.1 mol/L) corresponds to 27.28mg of C13H20N2O2 · hc1.
category
with procaine hydrochloride.
specification
(1)0.15g (2)lg
storage
light shielding, closed storage.
Last Update：2022-01-01 15:35:12
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Product Name: Procaine hydrochloride Request for quotationCAS: 51-05-8
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Mobile: +86-13651600618
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Wechat: 13651600618
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Product Name: Procaine Hydrochloride Visit Supplier Webpage Request for quotationCAS: 51-05-8
Tel: 0714-3999186
Email: 2853786052@qq.com
Mobile: 86+15671228036
QQ: 2853786052
Wechat: 15671228036
Product Name: Procaine hydrochloride Request for quotation
CAS: 51-05-8
Tel: 0086-551-65418684
Email: sales@tnjchem.com
info@tnjchem.com
Mobile: 0086 189 4982 3763
QQ: 2881500840
Wechat: 0086 189 4982 3763
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Tel: 0086-551-65418684
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